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Objective@#To investigate immune responses to influenza virus infections and the immunogenicity of trivalent split-virus influenza vaccine among elderly populations in Jiangbei District, Ningbo City, so as to provide the support for promoting influenza vaccination among elderly populations.@*Methods@#The elderly populations at ages of 60 years and older were recruited in Jiangbei District of Ningbo City from September to November, 2020, and the participants were assigned to the vaccination group and the control group according to vaccination intention. The titers of haemagglutination inhibition ( HI ) antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were measured using the micro HI test prior to vaccination and 30 days post-vaccination, and the protective rate, geometric mean titer ( GMT ) and seroconversion rate of antibodies were analyzed before and after vaccination.@*Results@#There were 290 participants in the vaccination group, including 132 men (45.52% ), and 290 controls, including 132 men ( 45.52% ). There were no significant differences between the vaccination group and the control group in terms of the protective rate or GMT of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV prior to vaccination ( P>0.05 ). Following vaccination, the protective rates of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were 98.62%, 94.14% and 88.28%, and the GMT of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV increased by 9.26, 6.19 and 10.09 folds, while the seroconversion rates of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were 78.62%, 68.28% and 71.38%, respectively. The protective rates, GMT and seroconversion rates of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were all significantly greater in the vaccination group than in the control group post-vaccination ( P<0.05 ). A lower increase was seen in the GMT of antibodies against the influenza virus BV among residents at ages of 80 years and older (increase by 7.91 folds) than among residents at ages of 70 to 79 years ( increase by 12.53 folds ) and 60 to 69 years (increase by 13.32 folds) in the vaccination group post-vaccination ( P<0.05 ), and the seroconversion rate of antibodies against the influenza virus BV was significantly lower in residents at ages of 80 years and older ( 62.57% ) than in those at ages of 70 to 79 years ( 83.33% ) ( P<0.05 ), while the positive conversion rate of antibodies against the influenza virus A ( H3N2 ) was significantly lower in residents at ages of 80 years and older ( 62.57% ) than in those at ages of 60 to 69 years ( 91.30% ) ( P<0.05 ).@*Conclusions@#Low-level immune responses are detected to antibodies against influenza virus A ( H3N2 ) and BV among elderly populations in Jiangbei District of Ningbo City, and trivalent split-virus influenza vaccine shows a high immunogenicity among elder populations. An emphases on improvements in coverage of influenza vaccination among elderly populations at ages of 60 to 69 years, and development of influenza vaccines with a higher protective efficacy for residents at ages of 80 years and older are recommended.
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Objective@#To explore the relationship between different temperature indicators and hospital admission for childhood pneumonia.@*Methods@#The hospital admissions for pneumonia in children aged 0-14 years and meteorological data in Ningbo from 2015 to 2017 were collected. A distributed lag non-linear model combined with a generalized linear model was employed to analyze the exposure-response relationships between different temperature indicators（daily average,minimum and maximum temperature;the first percentile as low temperature and the 99th percentile as high temperature）and hospital admission for childhood pneumonia.@*Results@#A total of 4 542 cases of childhood pneumonia were recruited. There were obvious seasonal fluctuations found in the inpatient volume of childhood pneumonia,which peaked in winter and bottomed in summer. After adjusting for potential confounding variables such as relative humidity,PM2.5,long term trend and seasonal trend,the results suggested that after exposed to whether low or high temperature,the inpatient volume of childhood pneumonia would increase. When the daily average temperature and daily minimum temperature were employed,the effect of high temperature on the increase of inpatient volume for childhood pneumonia was statistically significant and the cumulative relative risk for a lag of 0-7 days were 1.52（95%CI：1.04-2.23）and 1.59（95%CI：1.08-2.34）,respectively. When the daily maximum temperature was employed,the effect of low temperature on the increase of inpatient volume for childhood pneumonia was statistically significant and the cumulative relative risk for a lag of 0-7 days were 1.30（95%CI：1.02-1.66）.@*Conclusion@#Our findings suggested that an increased risk of hospital admission for childhood pneumonia was associated with both low and high temperature.
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Objective To understand the association between IKZF3 gene polymorphism and the risk of systemic lupus erythematosus (SLE) in Han ethnic group in southern China.Methods A case-control study was conducted among 213 SLE patients and 234 healthy controls.Venous blood samples were collected from them to measure single nucleotide polymorphism (SNP) in IKZF3 by using the method of restriction fragment length polymorphism (PCR-RFLP).Multivariate logistic analysis and generalized multifactor dimensionality reduction (GMDR) method were used under multiple genetic models (additive,dominant,recessive),to analyze the association between IKZF3 and SLE susceptibility or different clinical features and gene-gene interactions.In addition,bioinformatics analysis was also conducted.Results As for rs114509391,CA genotype might decrease the risk of SLE compared with AA genotype (OR=0.14,95％CI:0.03-0.56,P=0.006) and significant association was also observed under dominant model (OR=0.26,95％CI:0.09-0.81,P=0.02).Stratified analysis indicated that rs9635726 and rs9909593 were related to SLE onset.The study of clinical features showed that rs907091 was associated with both renal disorder (additive:OR=0.59,95％CI:0.35-0.98,P=0.043) and anti-SSB (dominant:OR=0.41,95％CI:0.18-0.96,P=0.040).rs9635726 GG and GA genotype might decrease the risk of anti-SSB compared with AA genotype (OR=0.37,95％ CI:0.16-0.88,P=0.025).In addition,bioinformatics analysis indicated that all the studied SNPs were functional.Conclusion IKZF3 rs114509391,rs9635726 and rs9909593 polymorphisms might be related to SLE susceptibility in Han ethnic group in southern China and rs9909593,rs907091 might be associated with renal disorder and anti-SSB.
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Objective To understand the association between IKZF3 gene polymorphism and the risk of systemic lupus erythematosus (SLE) in Han ethnic group in southern China.Methods A case-control study was conducted among 213 SLE patients and 234 healthy controls.Venous blood samples were collected from them to measure single nucleotide polymorphism (SNP) in IKZF3 by using the method of restriction fragment length polymorphism (PCR-RFLP).Multivariate logistic analysis and generalized multifactor dimensionality reduction (GMDR) method were used under multiple genetic models (additive,dominant,recessive),to analyze the association between IKZF3 and SLE susceptibility or different clinical features and gene-gene interactions.In addition,bioinformatics analysis was also conducted.Results As for rs114509391,CA genotype might decrease the risk of SLE compared with AA genotype (OR=0.14,95％CI:0.03-0.56,P=0.006) and significant association was also observed under dominant model (OR=0.26,95％CI:0.09-0.81,P=0.02).Stratified analysis indicated that rs9635726 and rs9909593 were related to SLE onset.The study of clinical features showed that rs907091 was associated with both renal disorder (additive:OR=0.59,95％CI:0.35-0.98,P=0.043) and anti-SSB (dominant:OR=0.41,95％CI:0.18-0.96,P=0.040).rs9635726 GG and GA genotype might decrease the risk of anti-SSB compared with AA genotype (OR=0.37,95％ CI:0.16-0.88,P=0.025).In addition,bioinformatics analysis indicated that all the studied SNPs were functional.Conclusion IKZF3 rs114509391,rs9635726 and rs9909593 polymorphisms might be related to SLE susceptibility in Han ethnic group in southern China and rs9909593,rs907091 might be associated with renal disorder and anti-SSB.
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Objective To investigate the clinical efficacy and its security on different kinds of atomization with mucosolvan and chymotrypsin in treating neonatal pneumonia.Methods Seventy-eight neonates who were diagnosed as neonatal pneumonia were divided into treatment group (n =39) and control group (n =39) by random number table method from Jul 2011 to Aug 2013 in our hospital.The control group was treated with chymotrypsin atomization,and the treatment group was treated with compression atomizing to inhale mucosolvan.The treatment effects of two groups were compared.Results Compared with the control group after treatment for 24 h,48 h,72 h,the treatment group had more significant increasing in PaO2,more decreasing in PaCO2 and more significant improvement in oxygenation index.There were statistical significances between the two groups(P ＜ 0.01).The treatment group spent shorter time in remission of symptoms,disappear of signs and hospital stay than that of the control group(P ＜ 0.05).In the treatment group,25 cases were markedly improved,9 cases were effective,5 cases were invalid.The effective rate was 87.2％.In the control group,14 cases were markedly effective,6 cases were effective,19 cases were invalid.The effective rate was 51.3％.The effective rate was significant difference between the two groups (P ＜ 0.01).The adverse drug reactions were not found in the process of treatment for two groups.Conclusion Mucosolvan atomizing inhalation has a better treatment effect than chymotrypsin.It can shorten the course of treatment,and is worth promoting the application.